Negligible nuclear FOXP3 expression in breast cancer epithelial cells compared with FOXP3-positive T cells

Raoul A Droeser; Ellen C Obermann; Anna Maria Wolf; Stephanie Wallner; Dominik Wolf; Alexandar Tzankov

doi:10.1016/j.clbc.2013.02.009

Negligible nuclear FOXP3 expression in breast cancer epithelial cells compared with FOXP3-positive T cells

Raoul A Droeser, Ellen C Obermann, Anna Maria Wolf, Stephanie Wallner (Co-author), Dominik Wolf, Alexandar Tzankov

Research output: Contribution to journal › Original Article › peer-review

Abstract

BACKGROUND: The presence of forkhead box protein 3 (FOXP3) in breast cancer cells is a matter of debate. We systematically analyzed expression of FOXP3 in 1574 breast carcinomas.

MATERIALS AND METHODS: For nuclear FOXP3 detection in epithelial breast cancer cells, tissue microarray technology was used. In addition, cultured breast cancer cell lines (ZR75-1, MCF7-WH, BT20, T47D, and SKBR3) were tested for FOXP3 expression using real-time polymerase chain reaction and flow cytometry for messenger RNA (mRNA) and protein quantification.

RESULTS: We could neither detect any significant levels of mRNA or protein expression of FOXP3 in human breast cancer cell lines, nor in breast cancer specimens. Weak nuclear staining for FOXP3 was detectable in 16 of 1574 breast cancer cases (1%) and was only seen in a small proportion of malignant cells. There was no difference in survival between patients with FOXP3-positive or -negative tumors.

CONCLUSION: FOXP3 does most likely not play a significant role in breast cancer biology, because it is only scarcely expressed in a very small proportion of breast cancer samples.

Original language	English
Pages (from-to)	264-70
Number of pages	7
Journal	CLINICAL BREAST CANCER
Volume	13
Issue number	4
DOIs	https://doi.org/10.1016/j.clbc.2013.02.009
Publication status	Published - Aug 2013
Externally published	Yes

Keywords

Adult
Aged
Aged, 80 and over
Biomarkers, Tumor/genetics
Breast Neoplasms/genetics
Carcinoma, Ductal, Breast/genetics
Carcinoma, Lobular/genetics
Cell Nucleus/genetics
Cytoplasm/metabolism
Epithelial Cells/metabolism
Female
Follow-Up Studies
Forkhead Transcription Factors/genetics
Humans
Immunoenzyme Techniques
Middle Aged
Neoplasm Invasiveness
Neoplasm Metastasis
Neoplasm Recurrence, Local/genetics
Neoplasm Staging
Prognosis
RNA, Messenger/genetics
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
T-Lymphocytes/metabolism
Tumor Cells, Cultured

Access to Document

10.1016/j.clbc.2013.02.009

Cite this

@article{4ae95ed189284e68b5758c404b056990,

title = "Negligible nuclear FOXP3 expression in breast cancer epithelial cells compared with FOXP3-positive T cells",

abstract = "BACKGROUND: The presence of forkhead box protein 3 (FOXP3) in breast cancer cells is a matter of debate. We systematically analyzed expression of FOXP3 in 1574 breast carcinomas.MATERIALS AND METHODS: For nuclear FOXP3 detection in epithelial breast cancer cells, tissue microarray technology was used. In addition, cultured breast cancer cell lines (ZR75-1, MCF7-WH, BT20, T47D, and SKBR3) were tested for FOXP3 expression using real-time polymerase chain reaction and flow cytometry for messenger RNA (mRNA) and protein quantification.RESULTS: We could neither detect any significant levels of mRNA or protein expression of FOXP3 in human breast cancer cell lines, nor in breast cancer specimens. Weak nuclear staining for FOXP3 was detectable in 16 of 1574 breast cancer cases (1\%) and was only seen in a small proportion of malignant cells. There was no difference in survival between patients with FOXP3-positive or -negative tumors.CONCLUSION: FOXP3 does most likely not play a significant role in breast cancer biology, because it is only scarcely expressed in a very small proportion of breast cancer samples.",

keywords = "Adult, Aged, Aged, 80 and over, Biomarkers, Tumor/genetics, Breast Neoplasms/genetics, Carcinoma, Ductal, Breast/genetics, Carcinoma, Lobular/genetics, Cell Nucleus/genetics, Cytoplasm/metabolism, Epithelial Cells/metabolism, Female, Follow-Up Studies, Forkhead Transcription Factors/genetics, Humans, Immunoenzyme Techniques, Middle Aged, Neoplasm Invasiveness, Neoplasm Metastasis, Neoplasm Recurrence, Local/genetics, Neoplasm Staging, Prognosis, RNA, Messenger/genetics, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes/metabolism, Tumor Cells, Cultured",

author = "Droeser, \{Raoul A\} and Obermann, \{Ellen C\} and Wolf, \{Anna Maria\} and Stephanie Wallner and Dominik Wolf and Alexandar Tzankov",

year = "2013",

month = aug,

doi = "10.1016/j.clbc.2013.02.009",

language = "English",

volume = "13",

pages = "264--70",

journal = "CLINICAL BREAST CANCER",

issn = "1526-8209",

number = "4",

}

TY - JOUR

T1 - Negligible nuclear FOXP3 expression in breast cancer epithelial cells compared with FOXP3-positive T cells

AU - Droeser, Raoul A

AU - Obermann, Ellen C

AU - Wolf, Anna Maria

AU - Wallner, Stephanie

AU - Wolf, Dominik

AU - Tzankov, Alexandar

PY - 2013/8

Y1 - 2013/8

N2 - BACKGROUND: The presence of forkhead box protein 3 (FOXP3) in breast cancer cells is a matter of debate. We systematically analyzed expression of FOXP3 in 1574 breast carcinomas.MATERIALS AND METHODS: For nuclear FOXP3 detection in epithelial breast cancer cells, tissue microarray technology was used. In addition, cultured breast cancer cell lines (ZR75-1, MCF7-WH, BT20, T47D, and SKBR3) were tested for FOXP3 expression using real-time polymerase chain reaction and flow cytometry for messenger RNA (mRNA) and protein quantification.RESULTS: We could neither detect any significant levels of mRNA or protein expression of FOXP3 in human breast cancer cell lines, nor in breast cancer specimens. Weak nuclear staining for FOXP3 was detectable in 16 of 1574 breast cancer cases (1%) and was only seen in a small proportion of malignant cells. There was no difference in survival between patients with FOXP3-positive or -negative tumors.CONCLUSION: FOXP3 does most likely not play a significant role in breast cancer biology, because it is only scarcely expressed in a very small proportion of breast cancer samples.

AB - BACKGROUND: The presence of forkhead box protein 3 (FOXP3) in breast cancer cells is a matter of debate. We systematically analyzed expression of FOXP3 in 1574 breast carcinomas.MATERIALS AND METHODS: For nuclear FOXP3 detection in epithelial breast cancer cells, tissue microarray technology was used. In addition, cultured breast cancer cell lines (ZR75-1, MCF7-WH, BT20, T47D, and SKBR3) were tested for FOXP3 expression using real-time polymerase chain reaction and flow cytometry for messenger RNA (mRNA) and protein quantification.RESULTS: We could neither detect any significant levels of mRNA or protein expression of FOXP3 in human breast cancer cell lines, nor in breast cancer specimens. Weak nuclear staining for FOXP3 was detectable in 16 of 1574 breast cancer cases (1%) and was only seen in a small proportion of malignant cells. There was no difference in survival between patients with FOXP3-positive or -negative tumors.CONCLUSION: FOXP3 does most likely not play a significant role in breast cancer biology, because it is only scarcely expressed in a very small proportion of breast cancer samples.

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Biomarkers, Tumor/genetics

KW - Breast Neoplasms/genetics

KW - Carcinoma, Ductal, Breast/genetics

KW - Carcinoma, Lobular/genetics

KW - Cell Nucleus/genetics

KW - Cytoplasm/metabolism

KW - Epithelial Cells/metabolism

KW - Female

KW - Follow-Up Studies

KW - Forkhead Transcription Factors/genetics

KW - Humans

KW - Immunoenzyme Techniques

KW - Middle Aged

KW - Neoplasm Invasiveness

KW - Neoplasm Metastasis

KW - Neoplasm Recurrence, Local/genetics

KW - Neoplasm Staging

KW - Prognosis

KW - RNA, Messenger/genetics

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - T-Lymphocytes/metabolism

KW - Tumor Cells, Cultured

U2 - 10.1016/j.clbc.2013.02.009

DO - 10.1016/j.clbc.2013.02.009

M3 - Original Article

C2 - 23829892

SN - 1526-8209

VL - 13

SP - 264

EP - 270

JO - CLINICAL BREAST CANCER

JF - CLINICAL BREAST CANCER

IS - 4

ER -

Negligible nuclear FOXP3 expression in breast cancer epithelial cells compared with FOXP3-positive T cells

Abstract

Keywords

Access to Document

Fingerprint

Cite this