Establishment of a Novel Short Tandem Repeat Typing Method for Exophiala dermatitidis

H Zoqi, D Schmidt, L Sedlacek, PM Rath, J Steinmann (Co-author), L Kirchhoff

Research output: Contribution to journalOriginal Articlepeer-review

1 Citation (Web of Science)

Abstract

The opportunistic black yeast-like fungus Exophiala dermatitidis frequently colonizes the respiratory tract of cystic fibroses (CF) patients. Additionally, it can cause superficial, systemic, and cerebral forms of phaeohyphomycoses. The objective of this study was to develop and apply a microsatellite or short tandem repeat (STR) genotyping scheme for E. dermatitidis. In total, 82 E. dermatitidis isolates from various geographic origins (environmental = 9, CF = 63, invasive isolates = 9, melanin-deficient mutant = 1) were included in this study. After next-generation sequencing of a reference strain and sequence filtering for microsatellites, six STR markers were selected and amplified in two multiplex PCR reactions. The included isolates were discriminated in a genetic cluster analysis using the Pearson algorithm to reveal the relatedness of the isolates. The E. dermatitidis isolates clustered on basis of both, their source and their origin. The invasive isolates from Asia were unrelated to isolates from CF. Nearly all environmental isolates were grouped separately from patients' isolates. The Simpson index was 0.94. In conclusion, we were able to establish a STR genotyping scheme for investigating population genomics of E. dermatitidis.
Original languageEnglish
Article number5
Number of pages9
JournalMYCOPATHOLOGIA
Volume189
Issue number1
DOIs
Publication statusPublished - Feb 2024

Keywords

  • Exophiala dermatitidis
  • Microsatellite PCR
  • Genotyping
  • Short tandem repeats
  • Cystic fibrosis
  • WANGIELLA-DERMATITIDIS
  • IDENTIFICATION

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