TY - JOUR
T1 - Complex patterns of ADAM12 mRNA and protein splice variants in the human placenta.
AU - Kokozidou, Maria
AU - Drewlo, S
AU - Bartz, C
AU - Raven, G
AU - Brandenburg, L O
AU - Wruck, C J
AU - Pufe, T
N1 - Kokozidou: Institute of Anatomy and Cell Biology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany; European Vascular Center Aachen-Mastricht, Department of Vascular Surgery, RWTH Aachen University Clinics, Pauwelsstrasse 30, 52074 Aachen, Germany
PY - 2011/1/26
Y1 - 2011/1/26
N2 - AimsTrophoblast fusion in the placenta is prerequisite to successful pregnancy and the pathological conditions related to it. The presence of syncytin-1, is not sufficient to explain the complete event and ADAM12 is a major co-player candidate. Via differential splicing, the ADAM12 gene produces a short and a long form, being the ADAM12-S and the ADAM12-L respectively.Methods and resultsWe investigated the localisation of both variants in the human placenta using whole mount in situ hybridisation, immunohistochemistry and Northern blotting in 1st (n=8) and 3rd (n=8) trimester placentae and in the case of NB in several cell lines. In Northern blotting, 1st and 3rd trimester placentae were positive for the ADAM12-S and Bewo, 293HEK, JAR, leucocytes, macrophages, 1st and 3rd trimester placentae were positive for ADAM12-L. In whole mount in situ hybridisation, the 1st and 3rd trimester placental syncytium was positive for both variants. In immunohistochemistry, ADAM12-L localised in the cytotrophoblast of both 1st and 3rd trimester placentae, while ADAM12-S localised in the complete syncytium, often including the cytotrophoblast.ConclusionThe different localisation of ADAM12-S and ADAM12-L indicates a possible different role making ADAM12-L a candidate for the fusion event, while the syncytial localisation of the ADAM12-S makes it a candidate for cell-cell and cell-matrix interactions between the placental syncytium and the maternal interface.
AB - AimsTrophoblast fusion in the placenta is prerequisite to successful pregnancy and the pathological conditions related to it. The presence of syncytin-1, is not sufficient to explain the complete event and ADAM12 is a major co-player candidate. Via differential splicing, the ADAM12 gene produces a short and a long form, being the ADAM12-S and the ADAM12-L respectively.Methods and resultsWe investigated the localisation of both variants in the human placenta using whole mount in situ hybridisation, immunohistochemistry and Northern blotting in 1st (n=8) and 3rd (n=8) trimester placentae and in the case of NB in several cell lines. In Northern blotting, 1st and 3rd trimester placentae were positive for the ADAM12-S and Bewo, 293HEK, JAR, leucocytes, macrophages, 1st and 3rd trimester placentae were positive for ADAM12-L. In whole mount in situ hybridisation, the 1st and 3rd trimester placental syncytium was positive for both variants. In immunohistochemistry, ADAM12-L localised in the cytotrophoblast of both 1st and 3rd trimester placentae, while ADAM12-S localised in the complete syncytium, often including the cytotrophoblast.ConclusionThe different localisation of ADAM12-S and ADAM12-L indicates a possible different role making ADAM12-L a candidate for the fusion event, while the syncytial localisation of the ADAM12-S makes it a candidate for cell-cell and cell-matrix interactions between the placental syncytium and the maternal interface.
KW - DISINTEGRIN-METALLOPROTEINASES
KW - CHORIOCARCINOMA CELLS
KW - TROPHOBLAST FUSION
KW - SYNCYTIAL FUSION
KW - GROWTH-FACTOR
KW - HERV-W
KW - EXPRESSION
KW - CANCER
KW - ENVELOPE
KW - RECEPTOR
U2 - 10.1016/j.aanat.2010.12.002
DO - 10.1016/j.aanat.2010.12.002
M3 - Original Article
C2 - 21330122
SN - 0940-9602
VL - 193
SP - 142
EP - 148
JO - ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
JF - ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
IS - 2
ER -