Complex patterns of ADAM12 mRNA and protein splice variants in the human placenta.

Maria Kokozidou* (Erstautor/-in), S Drewlo, C Bartz, G Raven, L O Brandenburg, C J Wruck, T Pufe

*Korrespondierende/r Autor/-in für diese Arbeit

Publikation: Beitrag in FachzeitschriftOriginalarbeitBegutachtung

13 Quellenangaben (Web of Science)

Abstract

AimsTrophoblast fusion in the placenta is prerequisite to successful pregnancy and the pathological conditions related to it. The presence of syncytin-1, is not sufficient to explain the complete event and ADAM12 is a major co-player candidate. Via differential splicing, the ADAM12 gene produces a short and a long form, being the ADAM12-S and the ADAM12-L respectively.Methods and resultsWe investigated the localisation of both variants in the human placenta using whole mount in situ hybridisation, immunohistochemistry and Northern blotting in 1st (n=8) and 3rd (n=8) trimester placentae and in the case of NB in several cell lines. In Northern blotting, 1st and 3rd trimester placentae were positive for the ADAM12-S and Bewo, 293HEK, JAR, leucocytes, macrophages, 1st and 3rd trimester placentae were positive for ADAM12-L. In whole mount in situ hybridisation, the 1st and 3rd trimester placental syncytium was positive for both variants. In immunohistochemistry, ADAM12-L localised in the cytotrophoblast of both 1st and 3rd trimester placentae, while ADAM12-S localised in the complete syncytium, often including the cytotrophoblast.ConclusionThe different localisation of ADAM12-S and ADAM12-L indicates a possible different role making ADAM12-L a candidate for the fusion event, while the syncytial localisation of the ADAM12-S makes it a candidate for cell-cell and cell-matrix interactions between the placental syncytium and the maternal interface.
OriginalspracheEnglisch
Seiten (von - bis)142-148
FachzeitschriftANNALS OF ANATOMY-ANATOMISCHER ANZEIGER
Jahrgang193
Ausgabenummer2
DOIs
PublikationsstatusVeröffentlicht - 26 Jan. 2011
Extern publiziertJa

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