TY - JOUR
T1 - A Polymer Bio-Photoelectrolytic Platform for Electrical Signal Measurement and for Light Modulation of Ion Fluxes and Proliferation in a Neuroblastoma Cell Line
AU - Ciocca, Manuela
AU - Marcozzi, Serena
AU - Mariani, Paolo
AU - Lacconi, Valentina
AU - Di Carlo, Aldo
AU - Cina, Lucio
AU - Rosato-Siri, Marcelo D.
AU - Zanon, Alessandra
AU - Cattelan, Giada
AU - Avancini, Enrico
AU - Lugli, Paolo
AU - Priya, Shashank
AU - Camaioni, Antonella
AU - Brown, Thomas M.
N1 - keine Aff. zu Bozen
PY - 2023/5
Y1 - 2023/5
N2 - Light control of living systems is an emerging field in bioelectronics, in regenerative medicine and cell-based therapy. Herein, the design of a semitransparent bio-photoelectrolytic platform for control of a neuroblastoma cell line via light pulses is laid out. The platform is based on conjugated polymer films interfaced with a biological electrolyte solution confined in a compact chamber. Human SH-SY5Y neuroblastoma cells are cultured for 3 days on the organic semiconductor and subjected to a pulsed light protocol. At the end of the culture time, proliferative activity of cells on the polymer film subjected to light pulses is reduced by 50% compared to the cultures kept in dark. An increase in intracellular Ca2+ level is observed, indicating a significant perturbation of the equilibrium potential of the cells. It is shown that the platform, in a sandwich-type closed architecture with two transparent electrodes, can provide a tool for the initial recording of bioelectrical photovoltage signals (mV) that can complement analysis with more sophisticated electrophysiological tools. Obtained results can pave the way to new noninvasive photomanipulation techniques to stimulate/control living cells and their proliferation through both optical and electrical stimulation and probes, for application in the fields of biosensing and biomedicine.
AB - Light control of living systems is an emerging field in bioelectronics, in regenerative medicine and cell-based therapy. Herein, the design of a semitransparent bio-photoelectrolytic platform for control of a neuroblastoma cell line via light pulses is laid out. The platform is based on conjugated polymer films interfaced with a biological electrolyte solution confined in a compact chamber. Human SH-SY5Y neuroblastoma cells are cultured for 3 days on the organic semiconductor and subjected to a pulsed light protocol. At the end of the culture time, proliferative activity of cells on the polymer film subjected to light pulses is reduced by 50% compared to the cultures kept in dark. An increase in intracellular Ca2+ level is observed, indicating a significant perturbation of the equilibrium potential of the cells. It is shown that the platform, in a sandwich-type closed architecture with two transparent electrodes, can provide a tool for the initial recording of bioelectrical photovoltage signals (mV) that can complement analysis with more sophisticated electrophysiological tools. Obtained results can pave the way to new noninvasive photomanipulation techniques to stimulate/control living cells and their proliferation through both optical and electrical stimulation and probes, for application in the fields of biosensing and biomedicine.
KW - Bioelectronics
KW - Calcium signaling
KW - Cell growth inhibition
KW - Cellular light stimulation
KW - neuroblastoma cell line SH-SY5Y
KW - Organic optoelectronics
KW - Organic semiconductors
UR - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=pmu_pure&SrcAuth=WosAPI&KeyUT=WOS:000928800700001&DestLinkType=FullRecord&DestApp=WOS_CPL
U2 - 10.1002/anbr.202200127
DO - 10.1002/anbr.202200127
M3 - Original Article
SN - 2699-9307
VL - 3
JO - Advanced Nanobiomed Research
JF - Advanced Nanobiomed Research
IS - 5
M1 - 2200127
ER -